A simple, accurate, precise and sensitive first order derivative spectrophotometric method was developed and validated for the determination of cefixime trihydrate and linezolid in combine tablet dosage forms. First order derivative spectrophotometric method is adopted to eliminate spectral interference. Cefixime trihydrate showed zero crossing points at 235.6 nm and 266.09 nm and 287.15 nm, while Linezolid showed zero crossing points at 223.8 nm and 251 nm. The method was found to be linear and obeys beer’s law in concentration range of 4-12 µg/ml for cefixime trihydrate and 12-36 µg/ml for linezolid. The developed method was validated as per ICH guidelines. The relative standard deviation (RSD) for repeatability, intraday and interday was found to be less than 2 %. This method is found suitable for day to day analysis of cefixime trihydrate and linezolid in combined tablet dosage form.
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